| Peer-Reviewed

Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years

Received: 18 June 2013     Published: 20 August 2013
Views:       Downloads:
Abstract

Researches of age-dependent changes of liver functions and their correction with the use of various biologically active substances are an important issue of modern biomedicine. Investigation of the influence of the enzymatic hydrolyzate of Chlorophytum comosum (L.) on the liver of rats at the age of two years at its experimental toxic damage showed that the substrate has expressed a pronounced hepatoprotective effect. Under its influence morphological changes in the rat liver induced by CCl4 are much less pronounced than in the controls. There are also less significant deviations from normal levels of Alanine transamilase (ALT), Alanine transaminase AST and Total Bilirubin in blood plasma. Information analysis of the state of the organ indicates that the level of adaptation and regeneration opportunities of the liver of rats treated with an enzymatic hydrolyzate of Chlorophytum comosum (L.) is significantly higher than at the liver of rats with experimental toxic liver damage without the use of studied substrate. Histopathological analysis confirmed the alleviation of liver damage in connection with use of enzymatic hydrolyzate of Chlorophytum comosum (L.)

Published in American Journal of Bioscience and Bioengineering (Volume 1, Issue 4)
DOI 10.11648/j.bio.20130104.11
Page(s) 49-54
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Liver, Hydrolizate, Hepatotoxicity, Hepatocyte, Chlorophytum Comosum (L.)

References
[1] D.A. Areshidze and M.A.Kozlova. The toxity studies of fermentaive hydrolyzate of Clorophytum comosum. Bulletin of the Moscow State Regional University,2009,2; 44-46.
[2] D.A. Areshidze, M.A. Kozlova and T.A. Snisarenko. Characteristics of rat's liver regeneration at the age of three months under the influence of Chlorophytum comosum enzymatic hydrolyzate. Bulletin of the Moscow State Regional University,20011,1; 28-32.
[3] T.H. Arnold and B.C. de Wet. Plants of southern Africa: names and distribution. Memoirs of the Botanical Surveys of South Africa 1993, 62 :968.
[4] S.E. Afton, B. Catron and J.A. Caruso. Elucidating the selenium and arsenic metabolic pathways following exposure to the non-hyperaccumulating Chlorophytum comosum (L.), spider plant. Journal of Experimental Botany 2009, 60; 4;1289-1297.
[5] G.G. Avtandilov. 2008.Medical Morphometry. Meditsina, Moscow. pp.298. [in Russian].
[6] J. Emerit, В. Samuel and N. Pavio. Cu-Zn super oxide dismutase as a potential antifibrotic drug for hepatitis C related fibrosis. Biomedicine & Pharmacotherapy,2006, 60,1; 1–4.
[7] M. Giese, U. Bauer-Doranth, S. Langebartels. H. Sandermann. Detoxification of Formaldehyde by the Spider Plant (Chlorophytum comosum (L.) L.) and by Soybean (Glycine max L.) Cell-Suspension Cultures. Plant Physiology1994,4: 1301-1309.
[8] A.J. Haagen-Smit. E.F. Darley, M. Zaitlin and H. Hull. Noble W. Investigation on injury to plants from air pollution in the Los Angeles area. Plant Physiology, 1952; 27:18-34.
[9] S.N. Hilmer, V.C. Cogger, R. Fraser, A.J. McLean, D. Sullivan and D.G. Le Couteur. Age-related changes in the hepatic sinusoidal endothelium impede lipoprotein transfer in the rat. Hepatology,2005;42: 1349–1354.
[10] G. Hsiao, M.Y. Shen, K.H. Lin, M.H. Lan, L.Y. Wu, D.S. Chou, C.H. Lin, C.H. Su, and J.R. Sheu, Antioxidative and hepatoprotective effects of Antrodia camphorata extract. Journal of Agricultural and Food Chemistry,2005, 51;11: 3302–3308.
[11] C.P. Kala. B.S.Dhyani and B.S. Sajwan. Developing the medicinal plants sector in northern India: challenges and opportunities. Journal of Ethnobiology and Ethnomedicine. 2006,6: 32-38.
[12] D.G. Le Couteur, V.C. Cogger, R.S. McCuskey, D.E. Cabo, B. Smedsrød, K.K. Sorensen, A. Warren and R. Fraser. Age-related changes in the liver sinusoidal endothelium: a mechanism for dyslipidemia. Ann N Y Acad Sci. 2007;1114:79-87.
[13] J.E.A. Leakey, H.C. Cunny and J Bazare. Effects of aging and caloric restriction on hepatic drug metabolizing enzymes in the Fisher 344 rat. I: the cytochrome P-450 dependent monooxygenase system. Mechanisms of Ageing and Development.1989;48(2); 145–155.
[14] M.D. Logsdon, Jr. Meyn and P.C .Besa. Apoptosis and the Bcl–2 gene family: patterns of expression and prognostic value in stage I and II follicular center lymphoma? Int J Radiat Oncol Biol Phys, 1999, 44: 19-29.
[15] H. Matsushita H. Kuwabara, H. Matsushita, S. Ishikawa and M.Mochizuki. Apoptosis Induced in Human Cell Lines by a Butanol Extract from Chlorophytum comosum (L.) Roots. Journal of Health Science, 2005,51: 341-345.
[16] F. Morisco, P.Vitaglione, D. Amoruso, B. Russo, V. Fogliano and N. Caporaso. Foods and liver health. Molecular Aspects of Medicine, 2008, 29,1-2; 144–150.
[17] K. Nagata, H. Suzuki and S. Sakaguchi. Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis. The Journal of Toxicological Sciences,2007,32,5; pp. 453–468.
[18] M. Petronijevic and K. Ilic. Associations of gender and age with the reporting of drug-induced hepatic failure: data from the VigiBase™. J Clin Pharmacol. 2013;53(4);435-443.
[19] D.L. Schmucker. Age-related changes in liver structure and function: Implications for disease ? Exp Gerontol. 2005 Aug-Sep;40(8-9):650-659.
[20] M. Thakur, S. Bhangava and V. Dixit. Immunomodulatory Activity of Chlorophytum borivilianum Sant. Evid Based Complement Alternative Medicine, 2007, 4; 419-423.
[21] K. Jones, L. Timchenko and N.A. Timchenko. The role of CUGBP1 in age-dependent changes of liver functions. Ageing Res Rev. 2012,11(4);442-9.
[22] C.F. van Bezooijen, F.R. de Leeuw-Israel and C.F. Hollander. On the role of hepatic cell ploidy in changes in liver function with age and following partial hepatectomy. Mech Ageing Dev. 1973;1(5);351-6.
[23] R.A. Wood, R. Orwell, M.D. Burchett, J. Tarran, and S.K. Brown Absorption of organic compounds in indoor air by commonly used indoor plants. Proceedings of Healthy Buildings,2000, 2; 125-130.
Cite This Article
  • APA Style

    David A. Areshidze, Lyudmila D. Timchenko, Maria A. Кozlova. (2013). Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years. American Journal of Bioscience and Bioengineering, 1(4), 49-54. https://doi.org/10.11648/j.bio.20130104.11

    Copy | Download

    ACS Style

    David A. Areshidze; Lyudmila D. Timchenko; Maria A. Кozlova. Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years. Am. J. BioSci. Bioeng. 2013, 1(4), 49-54. doi: 10.11648/j.bio.20130104.11

    Copy | Download

    AMA Style

    David A. Areshidze, Lyudmila D. Timchenko, Maria A. Кozlova. Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years. Am J BioSci Bioeng. 2013;1(4):49-54. doi: 10.11648/j.bio.20130104.11

    Copy | Download

  • @article{10.11648/j.bio.20130104.11,
      author = {David A. Areshidze and Lyudmila D. Timchenko and Maria A. Кozlova},
      title = {Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {1},
      number = {4},
      pages = {49-54},
      doi = {10.11648/j.bio.20130104.11},
      url = {https://doi.org/10.11648/j.bio.20130104.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20130104.11},
      abstract = {Researches of age-dependent changes of liver functions and their correction with the use of various biologically active substances are an important issue of modern biomedicine. Investigation of the influence of the enzymatic hydrolyzate of Chlorophytum comosum (L.) on the liver of rats at the age of two years at its experimental toxic damage showed that the substrate has expressed a pronounced hepatoprotective effect. Under its influence morphological changes in the rat liver induced by CCl4 are much less pronounced than in the controls. There are also less significant deviations from normal levels of Alanine transamilase (ALT), Alanine transaminase AST and Total Bilirubin in blood plasma. Information analysis of the state of the organ indicates that the level of adaptation and regeneration opportunities of the liver of rats treated with an enzymatic hydrolyzate of Chlorophytum comosum (L.) is significantly higher than at the liver of rats with experimental toxic liver damage without the use of studied substrate. Histopathological analysis confirmed the alleviation of liver damage in connection with use of enzymatic hydrolyzate of Chlorophytum comosum (L.)},
     year = {2013}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Protective Effect of Enzymatic Hydrolyzate of Chlorophytum Comosum (L.) against Experimental Toxic Liver Injury in Wistar Rats at the Age of Two Years
    AU  - David A. Areshidze
    AU  - Lyudmila D. Timchenko
    AU  - Maria A. Кozlova
    Y1  - 2013/08/20
    PY  - 2013
    N1  - https://doi.org/10.11648/j.bio.20130104.11
    DO  - 10.11648/j.bio.20130104.11
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 49
    EP  - 54
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20130104.11
    AB  - Researches of age-dependent changes of liver functions and their correction with the use of various biologically active substances are an important issue of modern biomedicine. Investigation of the influence of the enzymatic hydrolyzate of Chlorophytum comosum (L.) on the liver of rats at the age of two years at its experimental toxic damage showed that the substrate has expressed a pronounced hepatoprotective effect. Under its influence morphological changes in the rat liver induced by CCl4 are much less pronounced than in the controls. There are also less significant deviations from normal levels of Alanine transamilase (ALT), Alanine transaminase AST and Total Bilirubin in blood plasma. Information analysis of the state of the organ indicates that the level of adaptation and regeneration opportunities of the liver of rats treated with an enzymatic hydrolyzate of Chlorophytum comosum (L.) is significantly higher than at the liver of rats with experimental toxic liver damage without the use of studied substrate. Histopathological analysis confirmed the alleviation of liver damage in connection with use of enzymatic hydrolyzate of Chlorophytum comosum (L.)
    VL  - 1
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Center of Cell Biology and Applied Biotechnology, Moscow State Regional University, Moscow, Russian Federation

  • North Caucasian federal university, Stavropol, Russian Federation

  • Center of Cell Biology and Applied Biotechnology, Moscow State Regional University, Moscow, Russian Federation

  • Sections