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Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

Received: 18 December 2014     Accepted: 5 January 2015     Published: 14 January 2015
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Abstract

Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.

Published in American Journal of Bioscience and Bioengineering (Volume 2, Issue 6)
DOI 10.11648/j.bio.20140206.11
Page(s) 72-77
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Resin-Bond Magnets, Cell Culture, Magnetic Field, Gradient Magnetic Field, Adhesion, Proliferation

References
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Cite This Article
  • APA Style

    Tamara A. Ignatyeva, Victor N. Voyevodin, Anatoly N. Goltsev, Victoria V. Kiroshka, Alexander M. Bovda, et al. (2015). Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology. American Journal of Bioscience and Bioengineering, 2(6), 72-77. https://doi.org/10.11648/j.bio.20140206.11

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    ACS Style

    Tamara A. Ignatyeva; Victor N. Voyevodin; Anatoly N. Goltsev; Victoria V. Kiroshka; Alexander M. Bovda, et al. Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology. Am. J. BioSci. Bioeng. 2015, 2(6), 72-77. doi: 10.11648/j.bio.20140206.11

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    AMA Style

    Tamara A. Ignatyeva, Victor N. Voyevodin, Anatoly N. Goltsev, Victoria V. Kiroshka, Alexander M. Bovda, et al. Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology. Am J BioSci Bioeng. 2015;2(6):72-77. doi: 10.11648/j.bio.20140206.11

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  • @article{10.11648/j.bio.20140206.11,
      author = {Tamara A. Ignatyeva and Victor N. Voyevodin and Anatoly N. Goltsev and Victoria V. Kiroshka and Alexander M. Bovda and Valery V. Kalynovskii and Alexey N. Velikodny and Peter A. Kutsenko and Vladimir Golub and Yuri Dzhedzheria and Irina Sharai},
      title = {Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {2},
      number = {6},
      pages = {72-77},
      doi = {10.11648/j.bio.20140206.11},
      url = {https://doi.org/10.11648/j.bio.20140206.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20140206.11},
      abstract = {Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.},
     year = {2015}
    }
    

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    T1  - Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology
    AU  - Tamara A. Ignatyeva
    AU  - Victor N. Voyevodin
    AU  - Anatoly N. Goltsev
    AU  - Victoria V. Kiroshka
    AU  - Alexander M. Bovda
    AU  - Valery V. Kalynovskii
    AU  - Alexey N. Velikodny
    AU  - Peter A. Kutsenko
    AU  - Vladimir Golub
    AU  - Yuri Dzhedzheria
    AU  - Irina Sharai
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    N1  - https://doi.org/10.11648/j.bio.20140206.11
    DO  - 10.11648/j.bio.20140206.11
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
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    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20140206.11
    AB  - Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • Institute for Problems of Cryobiology and Cryomedicine NASU, 61015 Kharkov, Ukraine

  • Institute for Problems of Cryobiology and Cryomedicine NASU, 61015 Kharkov, Ukraine

  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine

  • Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine

  • Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine

  • Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine

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