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Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity

Received: 28 January 2017     Accepted: 13 February 2017     Published: 25 February 2017
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Abstract

Arsenic pollution is at present an emerging global crisis. Arsenic is a naturally occurring metalloid found in water, soil and air from natural and anthropogenic sources. Currently, Arsenic pollution has gained a burning global importance due to its toxic effects. Growth of major pulse crop Mungbean (Vigna radiata (L.)Wilczek) was affected right from seedling stage when treated with Sodium arsenate (Na2HAsO4.7H2O). With the increase in concentration of Sodium arsenate (5μM, 10μM and 20μM) significant decrease in seedling length, water content and primary leaf area was observed. The vital pigments like chlorophyll, caroteniod content as well as Hill activity reduced appreciably in sodium arsenate treated seedlings which indicates poor photosynthetic metabolism. Arsenate exposure also altered metabolism of main photosynthate, sucrose. Arsenate toxicity led to decrease in reducing and non reducing sugar content in the mungbean seedlings whereas starch content was elevated. Pretreatment of mungbean seeds with Phytohormone-GA3 and Kinetin could ameliorate Arsenate induced toxicity to different extent in terms of growth and sucrose metabolism. Thus, the use of GA3 and Kinetin may help to resist the arsenic toxicity in seedling stage, to some extent, in arsenic contaminated areas.

Published in American Journal of Bioscience and Bioengineering (Volume 5, Issue 1)
DOI 10.11648/j.bio.20170501.18
Page(s) 50-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Vigna radiata, Sodium arsenate, Phytohormones, Gibberellic acid, Kinetin, Sucrose Metabolism

References
[1] Rahman, M. M., Rahman, M. A., Maki, T. and Hasegawa, H. (2012). “Phytotoxicity of arsenate and salinity on early seedling growth of rice (Oryza sativa L.): A threat to sustainable rice cultivation in South and S E Asia”. Bull. Environ. Contam. Toxicol., 88: 695-702.
[2] Swarnakar, A. (2016) “Mitigation of Toxic effects of Sodium Arsenate on germination, seedling growth and amylolytic enzyme of Mungbean seedlings with Macronutrients, Micronutrients and Organic acids. Int.J.Curr.Microbiol.App.Sci., 5 (12): 151-160.
[3] Abedin, M. J. and Meharg, A. A. (2002) “Relative toxicity of arsenite and arsenate on Germination and early seedling growth of rice (Oryza sativa L.)”. Plant and soil, 243 (1): 57-66.
[4] Maitra, P. and Mukherji, S. (1979) “Effect of Lead on nucleic acid and protein contents of rice (Oryza sativa L.) seedlings and its interaction with IAA and GA3 in different plant systems”. Indian J. Exp. Biol., 17: 929-931.
[5] Chakrabarti, N. and Mukherji, S. (2003) “Alleviation of NaCl stress by pretreatment with phytohormones in Vigna radiata L.”Biol. Plant., 46: 589-594.
[6] Singh, H. and Dara, B. L. (1971) “Influence of presoaking of seeds with gibberellins and auxins on growth and yield attributes of wheat (Triticum aestivum L.) under high salinity, sodium adsorption ratio and boron levels”. Indian J. Agr. Sci., 41: 998-1003.
[7] Sawan, Z. M, Mohamed A. A, Sakr R. A, Tarrad. A. M (2000) “Effect of kinetin concentration and methods of application on seed germination, yield components, yield and fibre properties of the Egyptian cotton (Gossypium barbadense)”. Environmental and Experimental Botany,44 (1): 59–68.
[8] Mehta P. M., M. T. Jacob and B. V. N. Rao. (1974) “The effects of ascorbic acid, kinetin, coumarin and their combination on the germination, growth and enzyme catalase of Cajanus cajan var. selection 4-1A”. Geobios1: 53-59.
[9] Ogunwole A. A. and Otusanya, O. O. (2015) “Interaction of Aqueous Extracts of Tithoni adiversifolia, Chromolaena odorata and Kinetin Induced Growth and Accumulation of Chlorophyll in Hibiscus sabdariffa”. Inter. J. Agri. Innovations and Res., 3 (6): 1610-1619.
[10] Bhatnagar V. K. and G. R. Rastogi. (1980) “Effect of some growth regulators on Zea mays”. Geobios7: 183-184.
[11] Abdollah Bahrani (2015). “Kinetin and Abscisic acid effects on seed germination and seedlings growth of Maize (Zea mays L.) under salt stress condition”. ARPN Journal of Agricultural and Biological Science.10 (9): 351-357.
[12] Nandwal, A. S. and Bharati, S. (1982) “Effect of kinetin and IAA on growth, yield and nitrogen fixing efficiency of nodule in pea (Pisum sativum)”. Indian J. Plant Physiol. 25: 358-363.
[13] Merchan, F., de Lorenzo L., Rizzo, S. G., Niebel, A., Manyani, H., Frugier, F., Sousa C. and Crespi, M. (2007) “Identification of regulatory pathways involved in the reacquisition of root growth after salt stress in Medicago truncatula”. Plant J. 51: 1-17.
[14] Arnon, D. I. (1949). “Copper enzyme in isolated chloroplasts. Polyphenol Oxidase in Beta vulgaris.”Plant Physiol., 24: 1-15.
[15] Davies, B. H. (1965). “Analysis of Carotene pigments”, in Chemistry and Biochemistry of Plant Pigments. Goodwin, T. W. Ed. Academic press. New York: 489-531.
[16] Vishniac, W. (1957) “Methods for the study of Hill reaction”, in Methods of Enzymology”Colowick, S. P. and Kaplan, N. O. Eds. Academic press. New York, 4: 342-355.
[17] Miller G. L. (1972) “Use of dinitrosalicylic acid reagent for determination of reducing Sugars”. Anal. Chem., 31: 426-428.
[18] McCready, R. M., Guggolz, J., Silviera, V. and Owens, H. S. (1950). “Determination of Starch and amylase in vegetables”. Anal. Chem., 22: 1156-1158.
[19] Zidan, I., Azaizen, I. I. and Neuman, P. M. (1990) “Does salinity reduce growth in maize roots epidermal cells by inhibiting their capacity for cell wall acidification?”Plant Physiol., 93: 7-11.
[20] Choudhury, B, Mitra, S. and Biswas, A. K. (2010). “Regulation of sugar metabolism in rice (Oryza sativa L.) seedlings under arsenate toxicity and its improvement by phosphate”. Physiol. and Mol. Biol.of Plants. 16 (1): 59-68.
[21] Carbonell – Barrachina, A. A., Burlo – Carbonell, F., Mataix-Beneyto, J. (1995).“Arsenic uptake, distribution and accumulation in tomato plants: effect of arsenite on plant growth and yield”. J. Plant Nutr., 18 (6): 1237-1250.
[22] Stoeva, N., Berova, M. and Zlatev, Z. (2005) “Effect of arsenic on some physiological parameters in bean plants”. Biologia Plantarum., 49 (2): 293-296.
[23] Swarnakar, A. and Mukherji, S. (2005). “Amelioration of arsenic toxicity by phosphate salts in mungbean seedlings”. J. Environ. Biol., 26 (3): 551-555.
[24] Azizur, R. M., Hasegawa, H., Rahman, M. M., Islam, M. N., Miah, M. A. M. and Tasmen, A. (2007) “Effect of arsenic on photosynthesis, growth and yield of five widely cultivated rice (Oryza sativa L.) varieties in Bangladesh”. Chemosphere, 67: 1072-1079.
[25] Vidhu and Murty, Y. S. (1985) “Growth substance mediated chlorophyll changes in Tagetes erecta L”. Plant Physiol and Biochem., 12 (2): 93-96.
[26] Chakraborti, N. and Mukherji, S. (1994) “Effect of gibberellic acid and kinetin pretreatment as foliar spray on chlorophyll content, chlorophyllase enzyme activity, photosynthetic non- cyclic electron transport and CO2 uptake in C3 glycophyte mungbean under salt stress”. Plant Physiol. Biochem., 21: 59-64.
[27] Zaidi, P. H. and Singh, B. B. (1995). “Effect of growth regulators on IAA- oxidase and Peroxidase activity in soybean under salinity”. Indian J. Plant Physiol., 38 (4): 337-339.
[28] Sarin, M. N. and Narayanan, A. (1968) “Effect of soil salinity and growth regulators on germination and seedling metabolism of wheat”. Physiol. Plant., 21: 1201-1209.
[29] Acharya, U. T., Prakash, L. and Prathapasenan, G. (1990) “Effect of gibberellic acid on seedling growth and carbohydrate metabolism during germination of rice (Oryza sativa L., var. GR-3) under saline condition”. J. Agron. Crop. Sci., 165 (1): 6-13.
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    Arpita Swarnakar. (2017). Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity. American Journal of Bioscience and Bioengineering, 5(1), 50-55. https://doi.org/10.11648/j.bio.20170501.18

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    Arpita Swarnakar. Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity. Am. J. BioSci. Bioeng. 2017, 5(1), 50-55. doi: 10.11648/j.bio.20170501.18

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    AMA Style

    Arpita Swarnakar. Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity. Am J BioSci Bioeng. 2017;5(1):50-55. doi: 10.11648/j.bio.20170501.18

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  • @article{10.11648/j.bio.20170501.18,
      author = {Arpita Swarnakar},
      title = {Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {5},
      number = {1},
      pages = {50-55},
      doi = {10.11648/j.bio.20170501.18},
      url = {https://doi.org/10.11648/j.bio.20170501.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20170501.18},
      abstract = {Arsenic pollution is at present an emerging global crisis. Arsenic is a naturally occurring metalloid found in water, soil and air from natural and anthropogenic sources. Currently, Arsenic pollution has gained a burning global importance due to its toxic effects. Growth of major pulse crop Mungbean (Vigna radiata (L.)Wilczek) was affected right from seedling stage when treated with Sodium arsenate (Na2HAsO4.7H2O). With the increase in concentration of Sodium arsenate (5μM, 10μM and 20μM) significant decrease in seedling length, water content and primary leaf area was observed. The vital pigments like chlorophyll, caroteniod content as well as Hill activity reduced appreciably in sodium arsenate treated seedlings which indicates poor photosynthetic metabolism. Arsenate exposure also altered metabolism of main photosynthate, sucrose. Arsenate toxicity led to decrease in reducing and non reducing sugar content in the mungbean seedlings whereas starch content was elevated. Pretreatment of mungbean seeds with Phytohormone-GA3 and Kinetin could ameliorate Arsenate induced toxicity to different extent in terms of growth and sucrose metabolism. Thus, the use of GA3 and Kinetin may help to resist the arsenic toxicity in seedling stage, to some extent, in arsenic contaminated areas.},
     year = {2017}
    }
    

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    T1  - Enhancement Effect of Gibberellic Acid and Kinetin on Sucrose Metabolism in Mungbean Seedlings Under Arsenate Toxicity
    AU  - Arpita Swarnakar
    Y1  - 2017/02/25
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    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
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    AB  - Arsenic pollution is at present an emerging global crisis. Arsenic is a naturally occurring metalloid found in water, soil and air from natural and anthropogenic sources. Currently, Arsenic pollution has gained a burning global importance due to its toxic effects. Growth of major pulse crop Mungbean (Vigna radiata (L.)Wilczek) was affected right from seedling stage when treated with Sodium arsenate (Na2HAsO4.7H2O). With the increase in concentration of Sodium arsenate (5μM, 10μM and 20μM) significant decrease in seedling length, water content and primary leaf area was observed. The vital pigments like chlorophyll, caroteniod content as well as Hill activity reduced appreciably in sodium arsenate treated seedlings which indicates poor photosynthetic metabolism. Arsenate exposure also altered metabolism of main photosynthate, sucrose. Arsenate toxicity led to decrease in reducing and non reducing sugar content in the mungbean seedlings whereas starch content was elevated. Pretreatment of mungbean seeds with Phytohormone-GA3 and Kinetin could ameliorate Arsenate induced toxicity to different extent in terms of growth and sucrose metabolism. Thus, the use of GA3 and Kinetin may help to resist the arsenic toxicity in seedling stage, to some extent, in arsenic contaminated areas.
    VL  - 5
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Author Information
  • Department of Botany, Bangabasi College, University of Calcutta, West Bengal, India

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