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Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons

Received: 18 April 2022     Accepted: 7 May 2022     Published: 31 May 2022
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Abstract

A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0×104 cfu/g and Pennisetum hybridum (KgG) 3.2×105 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36±1°C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.

Published in American Journal of Bioscience and Bioengineering (Volume 10, Issue 3)
DOI 10.11648/j.bio.20221003.12
Page(s) 48-52
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Endophytes, Bacteria, Plants, Lablab purpureau, Pennisetum hybridum

References
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    Abdullahi Yusuf Bello, Abubakar Muazu Jodi, Abubakar Sanusi, Ridwan Abdulsalam Hussein, Asabe Danjuma. (2022). Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons. American Journal of Bioscience and Bioengineering, 10(3), 48-52. https://doi.org/10.11648/j.bio.20221003.12

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    ACS Style

    Abdullahi Yusuf Bello; Abubakar Muazu Jodi; Abubakar Sanusi; Ridwan Abdulsalam Hussein; Asabe Danjuma. Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons. Am. J. BioSci. Bioeng. 2022, 10(3), 48-52. doi: 10.11648/j.bio.20221003.12

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    AMA Style

    Abdullahi Yusuf Bello, Abubakar Muazu Jodi, Abubakar Sanusi, Ridwan Abdulsalam Hussein, Asabe Danjuma. Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons. Am J BioSci Bioeng. 2022;10(3):48-52. doi: 10.11648/j.bio.20221003.12

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  • @article{10.11648/j.bio.20221003.12,
      author = {Abdullahi Yusuf Bello and Abubakar Muazu Jodi and Abubakar Sanusi and Ridwan Abdulsalam Hussein and Asabe Danjuma},
      title = {Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {10},
      number = {3},
      pages = {48-52},
      doi = {10.11648/j.bio.20221003.12},
      url = {https://doi.org/10.11648/j.bio.20221003.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20221003.12},
      abstract = {A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0×104 cfu/g and Pennisetum hybridum (KgG) 3.2×105 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36±1°C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons
    AU  - Abdullahi Yusuf Bello
    AU  - Abubakar Muazu Jodi
    AU  - Abubakar Sanusi
    AU  - Ridwan Abdulsalam Hussein
    AU  - Asabe Danjuma
    Y1  - 2022/05/31
    PY  - 2022
    N1  - https://doi.org/10.11648/j.bio.20221003.12
    DO  - 10.11648/j.bio.20221003.12
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 48
    EP  - 52
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20221003.12
    AB  - A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0×104 cfu/g and Pennisetum hybridum (KgG) 3.2×105 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36±1°C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.
    VL  - 10
    IS  - 3
    ER  - 

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Author Information
  • Department of Basic Science and General Studies, Federal College of Forestry Mechanization, Afaka, Nigeria

  • Department of Microbiology, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Microbiology, Kebbi State University of Science and Technology, Aliero, Nigeria

  • Department of Basic Science and General Studies, Federal College of Forestry Mechanization, Afaka, Nigeria

  • Department of Basic Science and General Studies, Federal College of Forestry Mechanization, Afaka, Nigeria

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