American Journal of Bioscience and Bioengineering

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Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata

Received: 22 January 2024    Accepted: 4 February 2024    Published: 21 February 2024
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Abstract

Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild.

DOI 10.11648/j.bio.20241201.13
Published in American Journal of Bioscience and Bioengineering (Volume 12, Issue 1, February 2024)
Page(s) 12-23
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rapid Screening, Microfluidic Chip, Wasmannia auropunctata, Invasive Alien Ants

References
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  • APA Style

    Jiaying, W., Li, L., Yuanjing, W., Junxia, C. (2024). Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. American Journal of Bioscience and Bioengineering, 12(1), 12-23. https://doi.org/10.11648/j.bio.20241201.13

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    ACS Style

    Jiaying, W.; Li, L.; Yuanjing, W.; Junxia, C. Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. Am. J. BioSci. Bioeng. 2024, 12(1), 12-23. doi: 10.11648/j.bio.20241201.13

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    AMA Style

    Jiaying W, Li L, Yuanjing W, Junxia C. Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. Am J BioSci Bioeng. 2024;12(1):12-23. doi: 10.11648/j.bio.20241201.13

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  • @article{10.11648/j.bio.20241201.13,
      author = {Wang Jiaying and Liu Li and Wang Yuanjing and Cui Junxia},
      title = {Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {12},
      number = {1},
      pages = {12-23},
      doi = {10.11648/j.bio.20241201.13},
      url = {https://doi.org/10.11648/j.bio.20241201.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20241201.13},
      abstract = {Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata
    AU  - Wang Jiaying
    AU  - Liu Li
    AU  - Wang Yuanjing
    AU  - Cui Junxia
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    DO  - 10.11648/j.bio.20241201.13
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 12
    EP  - 23
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20241201.13
    AB  - Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild.
    
    VL  - 12
    IS  - 1
    ER  - 

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Author Information
  • Technical Center, Ningbo Customs, Ningbo, China; Ningbo Academy of Inspection and Quarantine, Ningbo, China

  • Ningbo Zhongsheng Product Inspection Co., LTD, Ningbo, China

  • Community Health Service Center of Jiangxia Street, Ningbo, China

  • Technical Center, Ningbo Customs, Ningbo, China; Ningbo Academy of Inspection and Quarantine, Ningbo, China

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